General Information

  • ID:  hor004717
  • Uniprot ID:  O23262
  • Protein name:  Protein RALF-like 32
  • Gene name:  RALFL32
  • Organism:  Arabidopsis thaliana (Mouse-ear cress)
  • Family:  Plant rapid alkalinization factor (RALF) family
  • Source:  Plant
  • Expression:  NA
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Arabidopsis (genus), Camelineae (tribe), Brassicaceae (family), Brassicales (order), malvids, rosids, Pentapetalae, Gunneridae, eudicotyledons, Mesangiospermae, Magnoliopsida (class), Spermatophyta, Euphyllophyta, Tracheophyta, Embryophyta, Streptophytina (subphylum), Streptophyta (phylum), Viridiplantae (kingdom), Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0005179 hormone activity
  • GO BP:  GO:0007267 cell-cell signaling; GO:0019722 calcium-mediated signaling
  • GO CC:  GO:0005576 extracellular region; GO:0048046 apoplast

Sequence Information

  • Sequence:  EQAHKLSYGALRRNQPACDGGKRGESYSTQCLPPPSNPYSRGCSKHYRCGRDS
  • Length:  53
  • Propeptide:  MEIKPSRIFSTITIFFLCLLLAHVTSKASSSSLCNGSVAECSSMVETEEMSVIMESWSSQRLTEEQAHKLSYGALRRNQPACDGGKRGESYSTQCLPPPSNPYSRGCSKHYRCGRDS
  • Signal peptide:  MEIKPSRIFSTITIFFLCLLLAHVTS
  • Modification:  NA
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Cell signaling peptide that may regulate plant stress, growth, and development. Mediates a rapid alkalinization of extracellular space by mediating a transient increase in the cytoplasmic Ca(2+) concentration leading to a calcium-dependent signaling events through a cell surface receptor and a concomitant activation of some intracellular mitogen-activated protein kinases.
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  18-31; 43-49
  • Structure ID:  AF-O23262-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004717_AF2.pdbhor004717_ESM.pdb

Physical Information

Mass: 679914 Formula: C244H383N83O79S4
Absent amino acids: FIMVW Common amino acids: SGR
pI: 9.33 Basic residues: 11
Polar residues: 24 Hydrophobic residues: 6
Hydrophobicity: -135.28 Boman Index: -17243
Half-Life: 1 hour Half-Life Yeast: 30 min
Half-Life E.Coli: >10 hour Aliphatic Index 27.74
Instability Index: 7907.92 Extinction Coefficient cystines: 6210
Absorbance 280nm: 119.42

Literature

  • PubMed ID:  12611624
  • Title:  Peptomics, Identification of Novel Cationic Arabidopsis Peptides with Conserved Sequence Motifs.